CollagenVAlpha-1ChainN-propeptide-VRdomain(COL5A1)MonoclonalAntibody
CollagenValpha-1chainisaproteinthatinhumansisencodedbytheCOL5A1gene.Thisgeneencodesanalphachainforoneofthelowabundancefibrillarcollagens.Fibrillarcollagenmoleculesaretrimersthatcanbecomposedofoneormoretypesofalphachains.TypeVcollagenisfoundintissuescontainingtypeIcollagenandappearstoregulatetheassemblyofheterotypicfiberscomposedofbothtypeIandtypeVcollagen.ThisgeneproductiscloselyrelatedtotypeXIcollagenanditispossIBLethatthecollagenchainsoftypesVandXIconstituteasinglecollagentypewithtissue-specificchaincombinations.
MutationsinthisgeneareassociatedwithEhlers-Danlossyndrome,typesIandII.
ThisantibodyreactswithhumancollagenValpha-1chainpropeptide.Theepitoperecognizedisinthevariableregion.
Immunoblotting:useat1-5ug/ml.Immunohistochemistry:Formalin-fixed,paraffin-embeddedtissue,heat-inducedantigenretrieval.Useat1-10ug/ml.
Stainingofhumanskinwith#90009.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Reconstituteindistilledwater.
Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Reconstitutedproductshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
