ORDERINGINFORMATION
CatalogNo.:42042(cloneFN30-8)Format:400ugin10mMPBS,pH7.4,1%BSA,lyophilized.BACKGROUND
Fibronectinisa440kDaglycoproteinoftheextracellularmatrixthatbindstointegrins,collagen,fibrin,andheparansulfateproteoglycans.Fibronectinplaysamajorroleincelladhesion,growth,migration,anddifferentiation,anditisimportantforprocessessuchaswoundhealingandembryonicdevelopment.Alteredfibronectinexpression,degradation,andorganizationhasbeenassociatedwithanumberofpathologies,includingcancerandfibrosis.
SPECIFICATIONSUMMARY
Antigen:HumanplasmafibronectinHostSpecies:MouseAntibodysubtype:IgG1Accessionno.:P02751,NP_001293058
Specificity:Thisantibodyrecognizesanepitopeinthecell-bindingdomainofhumanfibronectinandcaninhibitcelladhesion.APPLICATIONS
ELISA:1-10ug/mlwithimmobilizedantigen.
WesternBlot:1-10ug/ml,reducingornon-reducingconditions.
CellAdhesionInhibitionAssay:0.1-1.0ug/mlon10ug/mlfibronectin-coatedmicrowellsinhibitsadhesionoftrypsinizedNRKcells.
Immunohistochemistry:1-10ug/ml,paraffin-embeddedorfrozentissuesectionswithProteinaseKtreatmentforantigenretrieval.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
Dissolvelyophilizedantibodyin200ulofdistilledwater(finalconcentration2.0mg/ml).
STORAGEANDSTABILITY
Thestocksolution(2.0mg/ml)canbestoredinaliquotsat-20oCfor1yearorcanbestoredat4oCfor6monthsafteradding0.1%sodiumazide.Preparedilutionsofstocksolutionimmediatelybeforeuse.Donotstoredilutedstocksolution.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures
