ORDERINGINFORMATION

CatalogNo.:56270(cloneLy1C6)

Format:100uginPBS,pH7.4,purifiedbyProteinGaffinitychromatography,0.09%sodiumazide,50%glycerol.

BACKGROUND

Lysosome-associatedmembraneproteins(LAMP1andLAMP2)aremajorconstituentsofthelysosomalmembrane.Thestructuresofthesetwoproteinsarecloselyrelatedwith37%sequencehomology.Botharetransmembraneglycoproteinslocalizedprimarilyinlysosomesandlateendosomes.CellsurfaceLAMP1andLAMP2promoteadhesionofhumanperipheralbloodmononuclearcells(PBMC)tovascularendotheliumwhichsuggeststhattheyareinvolvedinadhesionofPBMCatsitesofinflammation.

SPECIFICATIONSUMMARY

Antigen:Ratliverlysosomemembranes.

HostSpecies:Mouse

AntibodyClass:IgG1

Specificity:Thisantibodyrecognizeshuman,rat,andhamsterLAMP1.

Accessionno.:NP_036989.1GeneID:25328

APPLICATIONS

Immunoblotting:Useat1ug/ml.Abandof

~120kDaisdetected.

Immunocytochemistry:Useat5-10ug/ml.

LAMP1detectedinratlivermicrosomes.ImmunofluorescentdetectionofLAMP1in

transfectedHeLacells.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

REFERENCE

LewisVetal.1985.JCellBiol100:1839-1847.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.