AMP-activatedproteinkinase(AMPK)isaheterotrimericproteinmadeupofacatalyticalphasubunitandregulatorybetaandgammasubunits.Therearetwodistinctgenesforthealphasubunit,alpha1andalpha2.AMPKalpha1istheserine/threoninekinasecatalyticsubunitoftheAMPK.AMPKisresponsIBLeforregulatingfattyacidandcholesterolsynthesis.
SPECIFICATIONSUMMARYAntigen:Syntheticpeptiderepresentingaportionoftheproteinencodedwithinexon8.HostSpecies:RabbitAccessionno.:BAA36547.1GeneID:5562Preservatives:0.09%sodiumazideSPECIFICITYThisantibodyreactswithhuman,mouse,ratandbovineAMPactivatedproteinkinase,alpha-1catalyticsubunit.Basedon100%sequenceidentity,thisantibodyispredictedtoreactwithChicken,Turkey,Dog,Horse,Rabbit,Guineapig,Pig,Panda,Orangutan,RhesusMonkey,Gorilla,Chimpanzee,WestIndianoceancoelacanth,Medakafish,Niletilapia,DuckbillplatypusandThree-spinedstickleback.
Antibodyisaffinitypurified.
Immunoblotting:useat0.2-2ug/ml
DetectionofAMPKalpha1inextractsof(1)bovineaorticendothelialcells,(2)rataorticsmoothmusclecells,(3)HepG2cells,and(4)humanaorticendothelialcellswith#13008at2µg/ml.
Immunohistochemistry:useat0.2-2ug/ml.
DetectionofAMPKalpha1inFFPEsectionofhumanmusclewith#13008at0.2ug/ml.
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
Thisantibodyisstableat2-8oCfor1year.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
