
AMP-activatedproteinkinase(AMPK)isaheterotrimericproteinmadeupofacatalyticalphasubunit,andaregulatorybetaandgammasubunit.Therearetwodistinctgenesforthealphasubunit,alpha1andalpha2.AMPKalpha2istheserine/threoninekinasecatalyticsubunitoftheAMPK.AMPKisresponsIBLeforregulatingfattyacidandcholesterolsynthesis.
Thisaffinity-purifiedantibodyreactswithhuman,mouse,ratandbovineAMPKalpha-2.Theepitoperecognizedisintheregionbetweenaa350and400ofAMPKalpha-2.Basedon100%sequenceidentity,thisantibodyispredictedtoreactwithMouse,Rabbit,Guineapig,Pig,Orangutan,RhesusMonkey,Gorilla,Chimpanzee,White-tufted-earmarmoset,Crab-eatingmacaque,Africanelephant,Chinesehamster,NakedmoleratandNorthernwhite-cheekedgibbon.
APPLICATIONSImmunoblotting:useat0.2-2ug/ml
DetectionofhumanandmouseAMPKalpha2inwholecelllysates(50µg)ofHeLa,293T,Jurkat,mouseRenca,andmouseTCMK-1cellswith#13010at0.4ug/ml.
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
Thisantibodyisstableat2-8oCfor1year.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.