ORDERINGINFORMATION
CatalogNo.:13040
Format:100ugantigen-affinitypurifiedIgGinTris-citrate/phosphatebuffer,pH7-8,0.09%sodiumazide.
BACKGROUND
AurorakinaseA,alsoknownasserine/threonine-proteinkinase6,isanenzymethatinhumansisencodedbytheAURKAgene.AuroraAisamemberofafamilyofmitoticserine/threoninekinasesassociatedwithimportantprocessesduringmitosisandmeiosis.AuroraAisactivatedbyoneormorephosphorylations,anditsactivitypeaksduringtheG2phasetoMphasetransitioninthecellcycle.AuroraAdysregulationhasbeenassociatedwithhighoccurrenceofcancer.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptiderepresentingaportionoftheproteinencodedwithinexon5.
Accessionno.:NP_003591.2
GeneID:6790
HostSpecies:Rabbit
Specificity:ThisantibodyreactswithhumanAurorakinaseA,andtheepitoperecognizedmapstoaregionbetweenresidues50-100ofhumanAurorakinaseA.Basedon100%sequenceidentitythisantibodyispredictedtoreactwithchimpanzee,crab-eatingmacaque,andNorthernwhite-cheekedgibbon.
APPLICATIONS
Immunoprecipitation:2-10ug/mglysate.
ImmunoprecipitationofhumanAuroraAfrom35S-Met-labeledwholecelllysateofcellstransfectedwithahumanAuroraAexpressionconstruct.DetectionbyautorADIography.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableat2-8°Cforone(1)yearfromdateofreceipt.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
