Thebeta-lactamantibiotics(penicillinsandcephalosporins)arethemostfrequentlyusedantimicrobialagents.Allofthebeta-lactamsarestructurallyrelatedthroughthepresenceofacorebeta-lactamring.Bacterialresistancetobeta-lactamscontinuestoincrease,primarilyduetomicrobialproductionofbeta-lactamases.Beta-lactamasescatalyzethehydrolysisofthebeta-lactambondwhichdestroysanti-bacterialactivity.BacteriathatproduceTEM-orSHV-typebeta-lactamaseshavepointmutationsinstructuralgenesthathaveextendedthesubstratespecificityofthesebeta-lactamases.Asaresult,manyofthebeta-lactamase-producingGram-negativebacteriahavebecomemulti-drugresistant.
Antigen:5’-His-taggedE.coli205TEM-1R+beta-lactamase
Sequence:MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
Accessionno.:P62593HostSpecies:MouseCloneno:8A5.A10AntibodySubtype:IgG1Specificity:ThisantibodyrecognizesTEM-1-typebeta-lactamases.Immunoblotting:useat10ug/ml.Predictedmolecularweight~29kDa.
ELISA:useat10-20ug/ml(optimizedforimmobilizedbeta-lactamaseat10ug/ml).Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
Thisproductisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
