ORDERINGINFORMATION

CatalogNo.:15721

Size:100ugor500ugPEG-purifiedantibodyinPBS,pH7.4.

BACKGROUND

Thebeta-lactamantibiotics(penicillinsandcephalosporins)arethemostfrequentlyusedantimicrobialagents.Allofthebeta-lactamsarestructurallyrelatedthroughthepresenceofacorebeta-lactamring.Bacterialresistancetobeta-lactamscontinuestoincrease,primarilyduetomicrobialproductionofbeta-lactamases.Beta-lactamasescatalyzethehydrolysisofthebeta-lactambondwhichdestroysanti-bacterialactivity.BacteriatheproduceTEM-orSHV-typebeta-lactamaseshavepointmutationsinstructuralgenesthathaveextendedthesubstratespecificityofthesebeta-lactamases.Asaresult,manyofthebeta-lactamase-producingGram-negativebacteriahavebecomemulti-drugresistant.Beta-lactamasefromEnterobactercloacaeisaserinebeta-lactamasewithsubstratespecificityforcephalosporins.

SPECIFICATIONSUMMARY

Antigen:TypeIVbetalactamasefromEnterobactercloacae.Predictedmolecularwt.~41kDa.

Sequence:

MMRKSLCCALLLGISCSALATPVSEKQLAEVVANTITPLMKAQSVPGMAVAVIYQGKPHYYTFGKADIAANKPVTPQTLFELGSISKTFTGVLGGDAIARGEISLDDAVTRYWPQLTGKQWQGIRMLDLATYTAGGLPLQVPDEVTDNASLLRFYQNWQPQWKPGTTRLYANASIGLFGALAVKPSGMPYEQAMTTRVLKPLKLDHTWINVPKAEEAHYAWGYRDGKAVRVSPGMLDAQAYGVKTNVQDMANWVMANMAPENVADASLKQGIALAQSRYWRIGSMYQGLGWEMLNWPVEANTVVEGSDSKVALAPLPVAEVNPPAPPVKASWVHKTGSTGGFGSYVAFIPEKQIGIVMLANTSYPNPARVEAAYHILEALQ

Accessionno.:P05364HostSpecies:MouseCloneno.:3E11.G3

AntibodySubtype:IgG3

Specificity:ThisantibodyrecognizesTypeIVbeta-lactamaseofEnterobactercloacae.

APPLICATIONS

ELISA:useat10-20ug/ml(optimizedforimmobilizedbeta-lactamaseat10ug/ml).Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.