ORDERINGINFORMATION

Catalogno.:56257-100

Format:100ugProteinA-purifiedantibodyinPBS,pH7.2,50%glycerol,0.09%sodiumazide.

BACKGROUND

Inmammalianspecies,biliverdinreductase(BVR)convertstetrapyrroletobilirubin.BVRisauniqueenzymeinthatithastwopHoptima(6.8and8.7)andusesdifferentcofactorsateachpHoptimum:NADHatpH6.8andNADPHatpH8.7.BVRdisplayspIandmolecularmassmicroheterogeneitywhicharelikelyaresultofpost-translationalmodifications.BVRisnotinactivatedbyheatshock,anditsmicroheterogenietyispreservedunderthermalstress.HumanBVRisalongerpolypeptidethantheratequivalent(41-42kDavs.33kDa),althoughhumanandratBVRsharesomeepitopesandshowimmunochemicalcross-reactivity.

SPECIFICATIONSUMMARY

Antigen:Recombinantratliverbiliverdinreductase.

Accessionno.:P46844

GeneID:116599

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Specificity:Thisantibodyrecognizeshuman,mouse,rat,porcine,andhamsterbiliverdinreductase.

APPLICATIONS

Immunoblotting:Useat1-5ug/ml.Abandof~33kDa(rat)and~41-42kDa(human)isdetected.

Immunohistochemistry:useat1-10ug/ml.

DetectionofbiliverdinreductaseinratembryoRat-2celllysate.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DetectionofbiliverdinreductaseinmousebrainpurKinjecellsafterischemicinjury.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze/thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures