ORDERINGINFORMATION

Catalognos.:13000-50(50ug)13000-200(200ug)

Format:ProteinA-purifiedantibodyinPBS,pH7.2,50%glycerol,0.09%sodiumazide,.

BACKGROUND

NADPH-cytochromeP450reductaseisamicrosomalcomponentessentialfortransferringreducingequivalentsfromNADPHtomicrosomalheme-oxygenaseisozymes(HO-1andHO-2),tothevariouscytochromeP450isozymesandtocytochromecandb5.CytochromeP450reductasehasbeenidentifiedinvariousspecieswithmolecularweightsintherangeof76-81kDa.ThisenzymecontainsashorthydrophobicN-terminusthatanchorsittotheendoplasmicreticulumandnuclearenvelope.

SPECIFICATIONSUMMARY

Antigen:NativeratcytochromeP450reductase.

Accessionno.:P00388

GeneID:127966

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Specificity:Thisantibodyrecognizeshuman,mouse,rat,rabbit,canine,bovine,porcine,guineapig,hamster,monkeyandsheepcytochromeP450reductase.

APPLICATIONS

Immunoblotting:Useat1-5ug/ml.Abandof~78kDaisdetected.

Detectionofcytochromep450reductaseinnativeratcytochromep450reductase(Lane2),humanlivermicrosomes(Lane3),ratlivermicrosomes(Lane4),mouselivermicrosomes(Lane5).

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidrepeatedfreeze/thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.