ORDERINGINFORMATION
Catalognos.:13000-50(50ug)13000-200(200ug)
Format:ProteinA-purifiedantibodyinPBS,pH7.2,50%glycerol,0.09%sodiumazide,.
BACKGROUND
NADPH-cytochromeP450reductaseisamicrosomalcomponentessentialfortransferringreducingequivalentsfromNADPHtomicrosomalheme-oxygenaseisozymes(HO-1andHO-2),tothevariouscytochromeP450isozymesandtocytochromecandb5.CytochromeP450reductasehasbeenidentifiedinvariousspecieswithmolecularweightsintherangeof76-81kDa.ThisenzymecontainsashorthydrophobicN-terminusthatanchorsittotheendoplasmicreticulumandnuclearenvelope.
SPECIFICATIONSUMMARY
Antigen:NativeratcytochromeP450reductase.
Accessionno.:P00388
GeneID:127966
HostSpecies:Rabbit
AntibodyClass:Polyclonal
Specificity:Thisantibodyrecognizeshuman,mouse,rat,rabbit,canine,bovine,porcine,guineapig,hamster,monkeyandsheepcytochromeP450reductase.
APPLICATIONS
Immunoblotting:Useat1-5ug/ml.Abandof~78kDaisdetected.
Detectionofcytochromep450reductaseinnativeratcytochromep450reductase(Lane2),humanlivermicrosomes(Lane3),ratlivermicrosomes(Lane4),mouselivermicrosomes(Lane5).
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidrepeatedfreeze/thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
