ORDERINGINFORMATION

CatalogNos.:13901(clone2A2-1B7)and13902(clone2A4-1G9)

Format:200ug/cloneinphosphate-bufferedsaline,pH7.4

BACKGROUND

Glutathionereductase,alsoknownasglutathione-disulfidereductase,isanenzymethatinhumansisencodedbytheGSRgene.Glutathionereductasecatalyzesthereductionofglutathionedisulfidetothesulfhydrylformglutathione(GSH),whichisacriticalmoleculeinresistingoxidativestressandmaintainingthereducingenvironmentofthecell.Theimportanceoftheglutathionepathwayandenzymesthataffectitisgaininganincreasedlevelofattention.Althoughglutathionereductasehasbeenanattractivetargetformanypharmaceuticals,therehavebeennosuccessfulglutathionereductaserelatedtherapeuticcompoundscreatedtodate.

SPECIFICATIONSUMMARY

Antigen:RecombinanthumanglutathionereductasepurifiedfromE.coliexpressingthehumanglutathionereductasegene(aa44-522).

Accessionno.:P00390,NP_000628

HostSpecies:Mouse

AntibodyClass:IgG1κ

Specificity:Theseantibodiesrecognizehumanglutathionereductase.Theydonotcross-reactwithyeastglutathionereductase.

APPLICATIONS

ELISA:TitrationofglutathionereductaseMAbswithglutathionereductaseat3ug/mlonthewells.

ELISAPairs:Bothoftheseantibodiesfunctionascaptureanddetectionantibodiesat1ug/ml.

Immunoblotting:useat0.5-1.0ug/ml.Abandof~57kDaisdetected.

#13902#13901

Lane1:mwstandards

Lane2:Recombinanthumanglutathionereductase

Lane3:Lysateofglutathionereductase-transfected293Tcells

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Theseantibodiesarestableforatleastone(1)yearat-20oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.