ORDERINGINFORMATION

Catalogno.:13060(cloneGTS-1)

Format:100uglyophilizedpurifiedantibodyin10mMPBS,pH7.4,1%BSA.

BACKGROUND

Heme-oxygenaseisanenzymethatcatalyzeshemecatabolismtoyieldbiliverdin,iron,andcarbonmonoxide(CO).Therearethreeisoformsofheme-oxygenase:HO-1,HO-2,andHO-3.HO-1andHO-2havebeenidentifiedasthetwomajorisoformsinmammals.HO-1,alsoknownasheatshockprotein32(Hsp32)isinducedbymostoxidativestressinducers,cytokines,inflammatoryagents,andheatshock.HO-1deficiencyappearstocausereducedstressdefense,apro-inflammatorytendency,susceptibilitytoatheroscleroticlesionformation,endothelialcellinjury,andgrowthretardation.Therefore,up-regulationofHO-1isoneofthemajordefensemechanismsagainstoxidativestress.

SPECIFICATIONSUMMARY

Antigen:MicrosomalfractionoftransformedmouseT-celltransfectedwithrathemeoxygenase-1(WR19LrHO-1).

Accessionno.:NP_036712

GeneID:24451

HostSpecies:Mouse

AntibodyClass:IgG1

Specificity:Thisantibodyrecognizeshuman,rat,andmouseHO-1.ItdoesnotrecognizerabbitHO-1anddoesnotcross-reactwithHO-2.

APPLICATIONS

Immunoblotting:useat5-10ug/ml.Abandof~33kDaisdetected.

DetectionofHO-1inhumansmallintestine.

DetectionofratHO-1with#13060at5ug/ml.

Immunohistochemistry:useat5-10ug/mlonhumanfrozenorparaffin-embeddedtissue.Antigenretrievalwith0.4ug/mlProteinaseK.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DetectionofHO-1inhumanpancreas.

DetectionofHO-1inhumanpancreaticcarcinoma.

DetectionofHO-1inhumanthyroidcarcinoma.

DetectionofHO-1inhumanlungcarcinoma.

RECONSTITUTIONINSTRUCTIONS

Dissolvelyophilizedantibodyin50ulofdistilledwater;finalconcentrationwillbe2mg/ml.Iffurtherdilutionisneeded,dilutethisstockwith10mMPBS,pH7.4+1%BSAimmediatelybeforeuse.

STORAGEANDSTABILITY

Stocksolutionof2mg/mlshouldbestoredforone(1)yearat-20oCinappropriatealiquotstoavoidmultiplefreeze-thawcyclesorat4oCfor6monthswiththeadditionof0.1%sodiumazide.Dilutedantibodyshouldnotbestored.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.