ORDERINGINFORMATION
Catalogno.:13061(clone1F12.A6)Format:100uginPBS(1mg/ml),pH7.4.PurifiedbyProteinGaffinitychromatography.BACKGROUND
Heme-oxygenaseisanenzymethatcatalyzeshemecatabolismtoyieldbiliverdin,iron,andcarbonmonoxide(CO).Therearethreeisoformsofheme-oxygenase:HO-1,HO-2,andHO-3.HO-1andHO-2havebeenidentifiedasthetwomajorisoformsinmammals.HO-1,alsoknownasheatshockprotein32(Hsp32)isinducedbymostoxidativestressinducers,cytokines,inflammatoryagents,andheatshock.HO-1deficiencyappearstocausereducedstressdefense,apro-inflammatorytendency,susceptibilitytoatheroscleroticlesionformation,endothelialcellinjury,andgrowthretardation.Therefore,up-regulationofHO-1isoneofthemajordefensemechanismsagainstoxidativestress.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptidecorrespondingtoaa1-30ofhumanHO-1.Accessionno.:P09601,NP_002124.1HostSpecies:MouseAntibodyClass:IgG1Specificity:Thisantibodyrecognizeshuman,mouse,andbovineHO-1.Itdoesnotcross-reactwithHO-2.APPLICATIONS
ELISA:useat1ug/mlwithHO-1onthesolidphase.
Immunoblotting:useat0.5-1ug/ml.Abandof~32kDaisdetected.
Immunofluorescence:useat5-10ug/ml
DetectionofHO-1informaldehyde-fixedHeLacells.
Positivecontrol:HumancelllinelysatessuchasA431,A549,HeLa,HepG2,Jurkat,orMCF7.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
