ORDERINGINFORMATION
CatalogNo.:13062(clone6B8-2F2)Format:100uginPBS(1mg/ml),pH7.4,0.1%sodiumazide,50%glycerol.PurifiedbyProteinGaffinitychromatography.BACKGROUND
Heme-oxygenaseisanenzymethatcatalyzeshemecatabolismtoyieldbiliverdin,iron,andcarbonmonoxide(CO).Therearethreeisoformsofheme-oxygenase:HO-1,HO-2,andHO-3.HO-1andHO-2havebeenidentifiedasthetwomajorisoformsinmammals.HO-1,alsoknownasheatshockprotein32(Hsp32)isinducedbymostoxidativestressinducers,cytokines,inflammatoryagents,andheatshock.HO-1deficiencyappearstocausereducedstressdefense,apro-inflammatorytendency,susceptibilitytoatheroscleroticlesionformation,endothelialcellinjury,andgrowthretardation.Therefore,up-regulationofHO-1isoneofthemajordefensemechanismsagainstoxidativestress.
SPECIFICATIONSUMMARY
Antigen:His-taggedratheme-oxygenase-1(HO-1)Accessionno.:NP_036712.1HostSpecies:MouseAntibodyClass:IgG1Specificity:Thisantibodyrecognizeshuman,mouse,andratHO-1.Itdoesnotcross-reactwithHO-2.APPLICATIONS
ELISA:confirmedwithantibodyat1-10ug/mlbindingto5ug/mlHO-1onthesolidphase.
Immunoblotting:confirmedwithantibodyat1.0-2.0ug/mland20ugofratkidneylysate.Abandof~31kDaisdetected.Positivecontrol:Ratkidneylysate.
Thesearerecommended
concentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
