ORDERINGINFORMATION

Catalogno.:13825(cloneS210-5)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

ThePHYHgenecodesfortheenzymephytanoyl-CoA2-hydroxylasewhichiscriticalforthenormalfunctionofperoxisomes.Onesubstancebrokendowninperoxisomesisphytanicacid,atypeoffattyacidobtainedprimarilyfrombeefanddairyproducts.Phytanoyl-CoA2-hydroxylaseisresponsIBLeforoneofthefirststepsinbreakingdownphytanicacidviaalpha-oxidation.Insubsequentsteps,additionalenzymesinperoxisomesandotherpartsofthecellfurtherprocessthiscompoundintosmallermoleculesthatareutilizedassourcesofenergy.Phytanoyl-CoA2-hydroxylasemayhaveotherfunctionsinadditiontoitsroleinbreakingdownphytanicacid;itappearstohelpdeterminethenumberofperoxisomeswithincellsandisinvolvedinregulatingtheiractivity.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa1-338(full-length)ofhumanPHYH.Thissequenceis78%identicalinratand78%identicalinmouse.

Accessionnos:NP_001032626.1,O14832

GeneID:5264

HostSpecies:Mouse

AntibodyClass:IgG1

Specificity:Thisantibodyrecognizeshuman,mouse,andratPHYH.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

~40kDaisdetected.

Immunofluorescence:useat10ug/ml.

DetectionofPHYHinratbrainlysatewith#13825at5ug/ml.

DetectionofPHYHinneuroblastomacelllineSK-N-BEwith#13825at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)PHYHstain.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.