ORDERINGINFORMATION
CatalogNo.:13001Format:100ul(1mg/ml)inPBSpH7.0,50%glycerol,and0.0.9%sodiumazide.Affinity-purifiedonantigen.BACKGROUND
Superoxidedismutase(SOD)isanendogenouslyproducedintracellularenzymethatcatalyzesthedismutationofthesuperoxiderADIcalO2-tooxygenandhydrogenperoxidewhicharethenmetabolizedtoH2OandO2bycatalaseandglutathioneperoxidase.SODsplayanimportantroleinantioxidantdefensemechanisms.ThreedifferentSODisoenzymesarefoundinmammaliancells:SOD1,SOD2,andSOD3.
SPECIFICATIONSUMMARY
Antigen:RatCu/ZnSODAccessionno.:P07632,NP_058746.1HostSpecies:RabbitSpecificity:Thisantibodydetectsa23kDaproteininhuman(anda19kDaproteininotherspecies)onSDS-PAGEimmunoblots,correspondingtothemolecularmassofCu/ZnSOD.Thisantibodyrecognizeshuman,mouse,rat,canine,porcine,rabbit,ovine,Xenopus,andbovineCu/ZnSOD.
APPLICATIONS
Immunoblotting:useat0.5-1.0ug/ml
DetectionofCu/ZnSODinamixtureofhumancelllines.
Positivecontrol:Ratbraintissueextract
Immunohistochemistry:useat1-10ug/ml
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
