ORDERINGINFORMATION

CatalogNo.:13003

Size:100ul(1mg/ml)inPBSpH7.0,50%glycerol,and0.09%sodiumazide.Affinity-purifiedonantigen.

BACKGROUND

Superoxidedismutase(SOD)isanendogenouslyproducedintracellularenzymethatcatalyzesthedismutationofthesuperoxiderADIcalO2-tooxygenandhydrogenperoxidewhicharethenmetabolizedtoH2OandO2bycatalaseandglutathioneperoxidase.SODsplayanimportantroleinantioxidantdefensemechanisms.ThreedifferentSODisoenzymesarefoundinmammaliancells:SOD1,SOD2,andSOD3.

SPECIFICATIONSUMMARY

Antigen:HumanMnSOD

Accessionno.:P04179,NP_000627.2

HostSpecies:Rabbit

Specificity:Thisantibodydetectsa25kDaproteinonSDS-PAGEimmunoblotscorrespondingtothemolecularmassofMnSOD.Thisantibodyrecognizeshuman,monkey,mouse,rat,bovine,canine,porcine,hamster,guineapig,rabbit,sheep,chicken,Drosophila,andXenopusMnSOD.

APPLICATIONS

Immunoblotting:useat0.2-0.5ug/ml.Positivecontrol:ratbraintissueextract

Immunohistochemistry:1-10ug/ml

DetectionofMnSODwithinthemusclefibersofLPS-injectedrats.(Left:Untreated,Right:treatedwith3mmol/kgN-acetylcysteine)

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20°C.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.