ORDERINGINFORMATION

CatalogNo.Size

18812F100ug

Format:1mg/mlconjugatedtofluoresceinisothiocyanate(FITC)inPhosphate-BufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

Apolyhistidine-tagconsistsofatleastsixhistidine(His)residues,oftenattheN-orC-terminusofaprotein.Polyhistidine-tagsareoftenusedforaffinitypurificationofpolyhistidine-taggedrecombinantproteinsexpressedinEscherichiacoliandotherprokaryoticexpressionsystems.

SPECIFICATIONSUMMARY

Antigen:6-His(HHHHHH)conjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.ThisantibodydoesnotrecognizeallN-terminal6-Histaggedproteins.

APPLICATIONS

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunofluorescence:useatadilutionof1:100-1:400.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.Shieldfromlight.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.