ORDERINGINFORMATION

CatalogNo.Size

18814P100ug

Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphate-BufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratiois4:1.

BACKGROUND

Apolyhistidine-tagconsistsofatleastsixhistidine(His)residues,oftenattheN-orC-terminusofaprotein.Polyhistidine-tagsareoftenusedforaffinitypurificationofpolyhistidine-taggedrecombinantproteinsexpressedinEscherichiacoliandotherprokaryoticexpressionsystems.

SPECIFICATIONSUMMARY

Antigen:6-His(HHHHHH)conjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.ThisantibodydoesnotrecognizeallN-terminal6-Histaggedproteins.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:30,000.

Detectionof6-Histaggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18814Pdiluted1:25,000.

ELISA:useatadilutionof1:10,000-1:100,000.

Immunocytochemistry:useatadilutionof1:200-1:500.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.