ORDERINGINFORMATION

CatalogNo.Size

18812A100ug

Format:0.1mg/0.4ml50%gelslurryinPhosphate-BufferedSaline(PBS),pH7.2,0.09%sodiumazide.Antibodycoupledtoagarosebeadsusingcyanogenbromide.

BACKGROUND

Apolyhistidine-tagconsistsofatleastsixhistidine(His)residues,oftenattheN-orC-terminusofaprotein.Polyhistidine-tagsareoftenusedforaffinitypurificationofpolyhistidine-taggedrecombinantproteinsexpressedinEscherichiacoliandotherprokaryoticexpressionsystems.

SPECIFICATIONSUMMARY

Antigen:6-His(HHHHHH)conjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.ThisantibodydoesnotrecognizeallN-terminal6-Histaggedproteins.

APPLICATIONS

Immunoprecipitation:use15-25ulgelslurryper0.1-1mgproteinlysateorextract.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.