ORDERINGINFORMATION

CatalogNo.Size

188161mg

18816-01100ug

Format:1mg/mlinPhosphate-BufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

AU1isanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.TheAU1sequencerepresentstheaminoacidsequenceDTYRYIderivedfromthemajorcapsidproteinofbovinepapillomavirus-1(BPV-1).

SPECIFICATIONSUMMARY

Antigen:AU1(DTYRYI)conjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

DetectionofAU1-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith

#18816diluted1:5,000.

ELISA:fordetection,useatadilutionof1:1,000-1:30,000;forcoatinguseat1:100-1:500.

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunoprecipitation:useat1-4ug/mglysate.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.