ORDERINGINFORMATION

CatalogNo.Size

188181mg

18818-01100ug

Format:1mg/mlinPhosphate-BufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

AU1isanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.TheAU1sequencerepresentstheaminoacidsequenceDTYRYIderivedfromthemajorcapsidproteinofbovinepapillomavirus-1(BPV-1).

SPECIFICATIONSUMMARY

Antigen:AU1(DTYRYI)conjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

DetectionofAU1-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith

#18818diluted1:25,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Immunoprecipitation:useat1-4ug/mglysate.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.