ORDERINGINFORMATION

CatalogNo.Size

18816P100ug

Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphate-BufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.

BACKGROUND

AU1isanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.TheAU1sequencerepresentstheaminoacidsequenceDTYRYIderivedfromthemajorcapsidproteinofbovinepapillomavirus-1(BPV-1).

SPECIFICATIONSUMMARY

Antigen:AU1(DTYRYI)conjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:30,000.

DetectionofAU1-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith

#18816Pdiluted1:5,000.

Immunocytochemistry:useatadilutionof1:200-1:500.

ELISA:useatadilutionof1:10,000-1:100,000.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.