ORDERINGINFORMATION

CatalogNo.Size

188261mg

18826-01100ug

Format:1mg/mlinPhosphateBufferedSaline,pH7.2,0.09%sodiumazide.

BACKGROUND

c-mycisanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.ThepeptidesequenceofthemyctagisEQKLISEEDL(Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu).Thec-myctagisapproximately1202Daltonsinatomicmassandincludes10aminoacids.

SPECIFICATIONSUMMARY

Antigen:Aminoacids410-419(EQKLISEEDL)ofhumanmycconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:25,000.

Detectionofc-myc-taggedproteinin200,200,or50ngofE.colilysatecontainingtaggedfusionproteinwith#18826diluted1:25,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunofluorescence:useatadilutionof1:100-1:400.

ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Detectionofc-myc-taggedplakoglobin(gamma-catenin)ontransientlytransfectedhumanmicrovascularendothelialcellswith#18826at1ug/ml.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.