ORDERINGINFORMATION

CatalogNo.Size

18830F100ug

Format:1mg/mlconjugatedtofluoresceinisothiocyante(FITC)inPhosphateBufferedSaline(PBS),0.2%BSA,0.09%sodiumazide.Fluorophore/antibodyratio=6.0.

BACKGROUND

Enterokinase,alsoknownasenteropeptidase,isaserineproteasethatcatalyzesthehydrolysisofpeptidebondsinproteinsbut,unlikeotherkinases,doesnotcatalyzetransferofphosphategroups.Enterokinasecleavesproteinsfollowingalysineatanenterokinasecleavagesite,orECS(Asp-Asp-Asp-Asp-Lys).Invivo,thiscleavageresultsintrypsin-dependentactivationofotherpancreaticzymogens,suchaschymotrypsinogen,proelastase,procarboxypeptidaseandprolipaseinthelumenofthegut.

SPECIFICATIONSUMMARY

Antigen:DDDDKconjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunofluorescence:useatadilutionof1:100-1:400.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.Shieldfromlight.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.