ORDERINGINFORMATION

CatalogNo.Size

18832P100ug

Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.

BACKGROUND

Enterokinase,alsoknownasenteropeptidase,isaserineproteasethatcatalyzesthehydrolysisofpeptidebondsinproteinsbut,unlikeotherkinases,doesnotcatalyzetransferofphosphategroups.Enterokinasecleavesproteinsfollowingalysineatanenterokinasecleavagesite,orECS(Asp-Asp-Asp-Asp-Lys).Invivo,thiscleavageresultsintrypsin-dependentactivationofotherpancreaticzymogens,suchaschymotrypsinogen,proelastase,procarboxypeptidaseandprolipaseinthelumenofthegut.

SPECIFICATIONSUMMARY

Antigen:DDDDKconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:30,000.

Immunocytochemistry:useatadilutionof1:200-1:500.

ELISA:useatadilutionof1:10,000-1:100,000.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofECS-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18832Pdiluted1:25,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.