ORDERINGINFORMATION

CatalogNo.Size

188381mg

18838-01100ug

Format:1mg/mlinPhosphateBufferedSaline(PBS),0.09%sodiumazide.

BACKGROUND

TheGlu-GlutagrepresentsthepeptidesequenceEYMPMEwhichisderivedfromthemiddleTantigenofmousepolyomavirus.Giventhesmallsizeofthispeptide,itisunlikelythatmodifyingproteinswithitwillaffectproteinstructureorfunction.

SPECIFICATIONSUMMARY

Antigen:Syntheticpeptidecorrespondingtoaa314-319(EYMPME)ofmiddleTantigenofmousepolyomavirusconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunoprecipitation:use1-4ug/mglysateELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofGlu-Glu-taggedproteininE.colilysatecontaining200,100,and50ngoftaggedproteinwith#18838diluted1:25,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.