ORDERINGINFORMATION
CatalogNo.CloneNo.Size
188033G10100ul
Format:1mg/mlProteinG-purifiedantibodyinPhosphateBufferedSaline,pH7.4.
BACKGROUND
AGST-tagisoftenusedtoseparateandpurifyproteinsthatcontaintheGST-fusionprotein.Thetagis220aminoacidswhich,comparedtotagssuchasthemyc-tag,isquitelarge.ItcanbefusedtoeithertheN-terminusorC-terminusofaprotein.ManycommerciallyavailablesourcesofGST-taggedplasmidsincludeathrombindomainforcleavageoftheGSTtagduringproteinpurification.
SPECIFICATIONSUMMARY
Antigen:GSTproteinofSchistosomajaponicumexpressedinE.coli.
HostSpecies:Mouse
Antibodysubtype:IgG1
Specificity:ThisantibodyrecognizesGST.
APPLICATIONS
Immunoblotting:useat0.2-2ug/ml.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DetectionofGST-taggedproteininE.colilysatewith#18803at0.2ug/ml.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
