ORDERINGINFORMATION

CatalogNo.Size

188441mg

18844-01100ug

Format:1mg/mlinPhosphateBufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

AGST-tagisoftenusedtoseparateandpurifyproteinsthatcontaintheGST-fusionprotein.Thetagis220aminoacidswhich,comparedtotagssuchasthemyc-tag,isquitelarge.ItcanbefusedtoeithertheN-terminusorC-terminusofaprotein.ManycommerciallyavailablesourcesofGST-taggedplasmidsincludeathrombindomainforcleavageoftheGSTtagduringproteinpurification.

SPECIFICATIONSUMMARY

Antigen:Glutathione-S-Transferase(GST)fromSchistosomajaponicum.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonGST.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunoprecipitation:use1-4ug/mglysate.ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofGST-taggedfusionproteinin0.1,0.3,and1.0ugof293celllysatewith18844

diluted1:15,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.