ORDERINGINFORMATION

CatalogNo.Size

18842P100ug

Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.

BACKGROUND

AGST-tagisoftenusedtoseparateandpurifyproteinsthatcontaintheGST-fusionprotein.Thetagis220aminoacidswhich,comparedtotagssuchasthemyc-tag,isquitelarge.ItcanbefusedtoeithertheN-terminusorC-terminusofaprotein.ManycommerciallyavailablesourcesofGST-taggedplasmidsincludeathrombindomainforcleavageoftheGSTtagduringproteinpurification.

SPECIFICATIONSUMMARY

Antigen:Glutathione-S-Transferase(GST)fromSchistosomajaponicum.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonGST.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:30,000.

Immunocytochemistry:useatadilutionof1:200-1:500.

ELISA:useatadilutionof1:10,000-1:100,000.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofGST-taggedfusionproteinin1.0,0.3,and0.1ugof293celllysatewith18842Pdiluted1:15,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.