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Functional_antibody >
QED Bioscience/Glutathione-S-Transferase (GST) HRP/18844P/1 Ea
ORDERINGINFORMATION
CatalogNo.Size
18844P100ug
Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.
BACKGROUND
AGST-tagisoftenusedtoseparateandpurifyproteinsthatcontaintheGST-fusionprotein.Thetagis220aminoacidswhich,comparedtotagssuchasthemyc-tag,isquitelarge.ItcanbefusedtoeithertheN-terminusorC-terminusofaprotein.ManycommerciallyavailablesourcesofGST-taggedplasmidsincludeathrombindomainforcleavageoftheGSTtagduringproteinpurification.
SPECIFICATIONSUMMARY
Antigen:Glutathione-S-Transferase(GST)fromSchistosomajaponicum.
HostSpecies:Rabbit
Specificity:Antibodywasaffinity-purifiedonGST.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:30,000.
Immunocytochemistry:useatadilutionof1:200-1:500.
ELISA:useatadilutionof1:10,000-1:100,000.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DetectionofGST-taggedfusionproteinin1.0,0.3,and0.1ugof293celllysatewith18844Pdiluted1:5,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
