ORDERINGINFORMATION

CatalogNo.Size

18850P100ug

Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.

BACKGROUND

TheHAepitopetagisderivedfromtheHA-moleculecorrespondingtoaminoacidsYPYDVPDYAofhumaninfluenzahemagglutinin.Itisfrequentlyusedasanepitopetaginexpressionvectors.ManyrecombinantproteinshavebeenengineeredtoexpresstheHAtag,whichdoesnotappeartointerferewiththebioactivityorthebiodistributionoftherecombinantprotein.

SPECIFICATIONSUMMARY

Antigen:HA(YPYDVPDYA)conjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:30,000.

Immunocytochemistry:useatadilutionof1:200-1:500.

ELISA:useatadilutionof1:10,000-1:100,000.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofHA-taggedfusionproteinin200,100,and50ngofE.colicelllysatewith18850Pdiluted1:25,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.