ORDERINGINFORMATION

CatalogNo.Size

188621mg

18862-01100ug

Format:1mg/mlinPhosphateBufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

TheT7tagisan11aminoacidpeptide(MASMTGGQQMG)derivedfromtheleadersequenceofT7bacteriophagegene10whichencodesaT7majorcapsidprotein.TheT7tagservesasataginmanyexpressionvectors,andantibodiesspecificfortheT7tagareusefulforidentifyingandisolatingT7-taggedproteins.

SPECIFICATIONSUMMARY

Antigen:T7(MASMTGGQQMG)conjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunoprecipitation:use1-4ug/mglysate.ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofT7-taggedfusionproteinin200,100,and50ngofE.colilysatewith#18862diluted1:25,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.