ORDERINGINFORMATION

CatalogNo.Size

18864P100ug

Format:1mg/mlantibodyconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.

BACKGROUND

TheT7tagisan11aminoacidpeptide(MASMTGGQQMG)derivedfromtheleadersequenceofT7bacteriophagegene10whichencodesaT7majorcapsidprotein.TheT7tagservesasataginmanyexpressionvectors,andantibodiesspecificfortheT7tagareusefulforidentifyingandisolatingT7-taggedproteins.

SPECIFICATIONSUMMARY

Antigen:T7(MASMTGGQQMG)conjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:30,000.

Immunocytochemistry:useatadilutionof1:200-1:500.

ELISA:useatadilutionof1:10,000-1:100,000.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofT7-taggedfusionproteinin200,100,and50ngofE.colilysatewith#18864Pdiluted1:5,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.