ORDERINGINFORMATION

CatalogNo.Size

188701mg

18870-01100ug

Format:1mg/mlinPhosphateBufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

TheV5epitopetagrepresentsaa95-108(GKPIPNPLLGLDST)derivedfromRNApolymerasealphasubunitofsimianvirus5.

SPECIFICATIONSUMMARY

Antigen:Syntheticpeptidecorrespondingtoaa95-108(GKPIPNPLLGLDST)ofRNApolymerasealphasubunitofsimianvirus5conjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofV5-taggedfusionproteinin200,100,and50ngofE.colilysatewith#18870diluted1:25,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

PRODUCTREFERENCES

TsurumiAetal.2011.PLoSGenetics7:e1002086.XiaFetal.2008.BloSBIOLOGy6:e128.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.