
ORDERINGINFORMATION
CatalogNo.Size
18868P100ug
Format:1mg/mlconjugatedtohorserADIshperoxidase(HRP)inPhosphateBufferedSaline(PBS),pH7.2,0.2%BSA,0.1%Pro-Clean400.Molarenzyme/antibodyratio=4:1.
BACKGROUND
TheV5epitopetagrepresentsaa95-108(GKPIPNPLLGLDST)derivedfromRNApolymerasealphasubunitofsimianvirus5.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptidecorrespondingtoaa95-108(GKPIPNPLLGLDST)ofRNApolymerasealphasubunitofsimianvirus5conjugatedtoKLH.
HostSpecies:Goat
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:30,000.
Immunocytochemistry:useatadilutionof1:200-1:500.
ELISA:useatadilutionof1:10,000-1:100,000.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DetectionofV5-taggedfusionproteinin200,100,and50ngofE.colilysatewith#18868Pdiluted1:25,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.