ORDERINGINFORMATION

CatalogNo.:30102

CloneNo.:M5G10

Format:250ugProteinG-purifiedantibodyinPBS,pH7.4

BACKGROUND

Thefibroblastgrowthfactor(FGF)familyisagroupofmulti-functionalpolypeptidesthatfunctioninembryonicdevelopment,tissuerepair,neuronalcellsurvival,cardiovasculardisease,andtumOrigenesis.TheBIOLOGicalactivitiesofFGFaremediatedbycellsurfacereceptorsthathavetyrosinekinaseactivity.FGFR-1isactivatedbyFGF-1andFGF-2.Inneoplasticcells,overexpressionofFGF-1,FGF-2andFGFreceptorsappeartocontributetocellulartrans-formationandproliferation.

SPECIFICATIONSUMMARY

Antigen:RecombinanthumanectodomainofFGFR-1βexpressedinE.colibeginningwithproline23;antigencontainedNH2-terminalgly-ser-pro-gly-ileandCOOH-terminalglu-phesequences.

HostSpecies:MouseAntibodySubclass:IgG1Accessionno.:P11362

Specificity:Thisantibodyreactswithbothα(denatured)andβisoforms.Epitopeiswithinthesequencehis241andval267betweenIgloopsIIandIII.EpitopeismaskedinundenaturedFGFr1α.

APPLICATIONS

Immunohistochemistry:10ug/ml,paraffin-embeddedtissue.

Immunoblotting:1ug/mlrecognizes10ngrecombinantbacterial,baculoviralornativeFGFR1.

DetectionofFGFR-1innormalsalivarygland.

3010430105301063010230103

DetectionofαandβisoformsinrecombinantFGFR-1expressedinSf9cellsby#30102alongwithotherQEDBioscienceFGFR-1MAbs.

Immunoprecipitation:native,recombinant,andligand-labeledFGFr1.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

PRODUCTREFERENCES

Xuetal.(1992)JBiolChem267:17792

Wangetal.(1995)JBiolChem270:10231

Myokenetal.(1996)JPathol178:429

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.