productdatasheet
CatalogNo.:30104(M2F12)
Size:250ugProteinG-purifiedantibodyinPBS,pH7.4
Antigen:RecombinanthumanectodomainofFGFr1αexpressedinE.colibeginningwithpro23;antigencontainedNH2-terminalgly-ser-pro-gly-ileandCOOH-terminalglu-phesequences.
HostSpecies:MouseAntibodyClass:IgG2aStABIlizers:None
Preservatives:None.Availableonrequest.
30104reactswiththeNH2-terminusofuniqueNH2-terminalIgloopofFGFr1.Epitopeiswithinthesequencebetweenglu30andala74ofFGFr1α.Reactswithhuman,rat,andmousereceptor.Otherspeciesuntested.
Westernblotting:at1ug/mlrecognizes
10ngrecombinantbacterial,baculo-viral,ornativeFGFr1.
APPLICATIONS(CONT.)Immunoprecipitation:native,nuclear,recombinant,andligand-labeledFGFr1.
Immunohistochemistry:frozensectionsofhumanglioblastomas,para-formaldehyde-fixedcellsandnuclei,humanmyocardialbiopsies.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Theseantibodiesarestableforatleastone
(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Xuetal.(1992)JBiolChem267:17792;Proudovskyetal.(1994)JBiolChem269:31720;Morrisonetal.(1994)CancerRes54:2794;Zhaoetal.(1994)JClinInvest
94:992;Fengetal.(1996)Biochim
BiophysActa1310:67.
Forresearchonly.Notforuseintherapeuticordiagnosticprocedures
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