TumorNecrosisFactor-Receptor1(TNF-R1)PolyclonalAntibody
CatalogNumber | Quantity |
11062-200 | 200ug |
CatalogNumber | Quantity |
11062-200 | 200ug |
ORDERINGINFORMATION
SPECIFICATIONSUMMARY
Antigen:AsyntheticpeptidecorrespondingtoaportionofmouseTNF-R1.Thissequenceiscompletelyconservedinratandhuman.Accessionno.P25118.HostSpecies:Rabbit.Format:Peptideaffinitypurified.Formulation:InPBScontaining50%glyceroland0.09%sodiumazide.Tumornecrosisfactoralpha(TNF-α),alsoknownascachetin,isamemberoftheTNFsuperfamilyofcytokinesthatisapotentlymphoidfactorwitheffecsonawiderangeoftargetcells.TNFexertsproinflamatorysignalsvabindingandinducingtrimerizationofTNF-receptor1(TNF-R1)expressedonmostnormalandtransformedcells.TNFsignalingregulateshematopoiesis,differentiation,endothelialcellactivation,apoptosis,lipidmetabolism,tumorprogression,andimmunesurveillance.
DysregulationofTNForitsreceptorsisimplicatedinnumerousdiseasesincludingcancer,osteoporosis,autoimmunedisease,diabetes,andatherosclerosis.
Thisantibodydetectsa55kDaproteinonheatshockedHeLacelllysateSDS-PAGEimmunoblots,correspondingtothemolecularweightofTNF-R1.Thisantibodyreactswithhuman,mouse,rat,hamster,bovine,rabbit,monkey,sheep,canine,pig,Drosophila,Xenopus,andyeastTNF-R1.
APPLICATIONSWesternBlot:1ug/ml
WesternblotanalysisofTNF-R1:Lane1MWMarkers,Lane2Heat-shockedHeLacelllysate,Lane3Jurkatcelllysate.
Immunohistochemistry(paraffin):1-10ug/ml
Humanspleentissuestainedwith#11062-200at10ug/ml.
Immunoprecipitation:12.5ug/ml
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Storefrozenproductatorbelow-20°C.Thawedproductmaybestoredfor2-4weeksat4°C.Foroptimalstorage,aliquotandstoreat-20°C.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
