ORDERINGINFORMATION
CatalogNo.:11148(clone6E3-2C3)Format:100uginPBS(1mg/ml),pH7.4,0.1%sodiumazide,50%glycerol.PurifiedbyProteinGaffinitychromatography.BACKGROUND
Grp170isamajorchaperone/stressproteinresidentintheER.Itisdistantlyrelatedinsequencetobothhsp110andhsp70families,butrepresentstheGrp170stressproteinfamily.StudieshavesuggestedthatGrp170isanimportantelementoftheproteinprocessingmachineryoftheER.UnlikeHsp110andHsp70,Grp170hastwodistinctchaperoningdomains;aclassicβ-sheetpeptidebindingdomain,withsomeCOOH-terminalsequence,andaCOOH-terminalα-helicaldomain.LikeothermembersoftheHsp70superfamily,Grp170canelicitanti-tumorimmuneresponsesmostlikelybychaperoningantigenicpeptideandbindingtoreceptor(s)onantigen-presentingcells(APC).
SPECIFICATIONSUMMARY
Antigen:SyntheticpeptidesequencederivedfromhumanGrp170(accessionno.NP_006389.3)HostSpecies:MouseAntibodyClass:IgG2bSpecificity:Thisantibodyrecognizeshuman,mouse,andratGrp170(170kDa).APPLICATIONS
ELISA:confirmedwithantibody(1-10ug/ml)bindingtoGrp170at5ug/mlonthesolidphase.
Immunoblotting:confirmedwithantibodyat1.0-2.0ug/ml.Abandof170kDaisdetected.Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:HEK293celllysate.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
