CatalogNo.:11135(clone6H4-2G7)Size:100uginPBS(2mg/ml),pH7.4,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

GRP78isaubiquitouslyexpressed,78kDaglucose-regulatedproteincommonlyreferredtoasanimmunoglobulinchainbindingprotein(BiP).Categorizedasstressresponseproteins,BiPsplayanimportantroleinfoldingandassemblyofnascentproteinsandinscavengingofmisfoldedproteinsintheendoplasmicreticulumlumen.

TranslationofBiPisdirectedbyaninternalribosomalentrysite(IRES)inthe5-nontranslatedregionofBiPmRNA.BiPIRESactivityincreaseswhencellsareheat-stressed.GRP78isalsoimportantinmaintenanceofcellhomeostasisandthepreventionofapoptosis.LevelsofGRP78proteinarereducedinbrainsofAlzheimer’sdiseasepatients.

Antigen:His-taggedhumanGRP78(accessionno.NP_001156906.1HostSpecies:Mouse

AntibodyClass:IgG1

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

ThisantibodyrecognizeshumanGRP78.

ELISA

Immunoblotting:useat0.5-1.0ug/ml.Abandof~78kDaisdetected.

Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:HeLacelllysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.