CatalogNo.:11144(clone3C5-1A4)Size:100uginPBS(2mg/ml),pH7.4,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

GRP78isaubiquitouslyexpressed,78kDaglucose-regulatedproteincommonlyreferredtoasanimmunoglobulinchainbindingprotein(BiP).Categorizedasstressresponseproteins,BiPsplayanimportantroleinfoldingandassemblyofnascentproteinsandinscavengingofmisfoldedproteinsintheendoplasmicreticulumlumen.

TranslationofBiPisdirectedbyaninternalribosomalentrysite(IRES)inthe5-nontranslatedregionofBiPmRNA.BiPIRESactivityincreaseswhencellsareheat-stressed.GRP78isalsoimportantinmaintenanceofcellhomeostasisandthepreventionofapoptosis.LevelsofGRP78proteinarereducedinbrainsofAlzheimer’sdiseasepatients.

Antigen:Full-lengthrecombinantratGRP78.

Accessionno.:NP-037215.1

GeneID:25617

HostSpecies:Mouse

AntibodyClass:IgG1

Thisantibodyrecognizeshuman,mouse,andratGRP78.

ELISA

Immunoblotting:useat1ug/ml.Abandof

~78kDaisdetected.

DetectionofGRP78with#11144at1ug/ml.

Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:HEK293celllysate.

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.