productdatasheet
Hsp70genes,membersofamultigenefamily,encodeheat-inducIBLe70kDaheat-shockproteinsthatfunctionasmolecularchaperones.Theyhavebeenidentifiedinmostorganellesofeukaryoticcellsaswellasinbacteria.TheN-terminusofHsp70bindsATPwithhighaffinityandtheC-terminusbindsproteinsandpolypeptides.Whencellsaresubjectedtometabolicstress,Hsp70isexpressed.Hsp70shares
>90%sequencehomologywithHsc70.ConstitutivelyexpressedHsc70formsastablecomplexwiththehighlyinducibleHsp70incellsfollowingheatshock.ThisinteractionisregulatedbyATP.ResearchonHsc70suggeststhatitplaysaroleinfacilitatingtherecoveryofcentrosomalstructureandfunctionafterheatshock.
Thisantibodyrecognizeshuman,mouse,andratHsc70.Otherspecieshavenotbeentested.Itdoesnotcross-reactwithHsp70.
Immunoblotting:useat1ug/ml.Abandof
~73kDaisdetected.
ELISA:useat1-10ug/mlImmunohistochemistry:useat1-10ug/ml.Immunoprecipitation:1-10ug/ml.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:HeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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