productdatasheet
Heatshockfactor1(HSF1)isaheatshocktranscriptionfactorthatactivatesthetranscriptionofgenesencodingproductsrequiredforproteinfolding,processing,targeting,degradation,andfunction.Up-regulationofexpressionofheatshockproteinsinresponsetostressoccursattheleveloftranscriptionthroughaheatshockelementandaHSFtranscriptionfactor.
AminoacidsequencesformostHSFsarehighlyconserved.ADNAbindingdomainisattheN-terminus,hydrophobicrepeats(essentialtotheformationofactivetrimers)areadjacenttothisbindingdomain,andanothershorthydrophobicrepeat(necessaryforsuppressionoftrimerization)occurstowardtheC-terminus.Inhighereukaryotes,HSF1isdistributeddiffuselyinthecellcytoplasmandnucleusinunstressedcells.Onexposuretoheatshockorotherstresses,HSF1localizestodiscretenucleargranules;onrecoveryfromstress,itreturnstoadiffusenuclear-cytoplasmicdistribution.
Thisantibodyrecognizesan~85kDaprotein(inactiveform)inunstressedcelllysatesandan~95kDaprotein(activeform)inheat-shockedcelllysatesofhuman,mouse,rat,rabbit,bovine,guineapig,hamster,andmonkey.
Immunoblotting:useat1-2ug/ml.Abandof
~85and/or~95kDaisdetected.Immunocytochemistry:useat10ug/ml.Immunoprecipitation:useat12.5ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:HeLacelllysate
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Cotto,Jetal.1997JCellScience110:2925-2934.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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