ADVANCEDRESEARCHTECHNOLOGIES
CatalogNo.:11133(clone3C12.H11)Size:100ugProteinG-purifiedantibodyinPBS,pH7.4,50%glycerol,0.09%sodiumazide.
Hsp22(HspB8)isamemberofthesmallheatshockproteinsuper-family.LikemostothersHsps,Hsp22ispredominantlytranscribedinskeletalmuscleandheartaswellasplacenta.ItisamonomericproteinwhichinteractswithHspB1.Itdisplaystemperature-dependentchaperoneactivity.Hsp22hastwobindingdomains(N-andC-terminal)thatarespecificfordifferentbindingpartnersandhastheABIlitytobinditselfandothersHsps.Itschaperone-likeactivityisimportanttothefunctionofHsp22invariousprocessesincludingproliferation,apoptosis,andmacroautophagy.
HostSpecies:Mouse
Antigen:His-taggedrecombinanthumanHsp22
AntibodyClass:IgG1
Preservatives:0.09%sodiumazide
Thisantibodyrecognizeshuman,mouse,andratHsp22.ItdetectsendogenousandexogenousHsp22inmonomeric,dimeric,andtetrameric(weak)formsinWesternblots.
ExogenousHsp22isdetectedindimericform.Itdoesnotcross-reactwithalphacrystallin.
Immunoblotting:useat1-10ug/ml.Abandof~22kDa(monomeric)isdetected.Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Wholerattissueextract.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
10919TechnologyPlace,SuiteCSanDiego,California92127Tel.(800)929-2114/(858)675-2405Fax(858)592-1509Emailinfo@qedbio.comwww.qedbio.com
