
productdatasheet
MembersofthehumanHsp40/DnaJproteinfamilyfeatureaJdomain(namedforthebacterialDnaJprotein).TheJdomainspansthefirst75N-terminalaminoacidsandisseparatedfromtheC-terminusbyaglycine-phenylalanine-rechdomain.TheHsp40/DnaJproteinsplaymanydifferentrolesincellularprocessessuchasfolding,translocation,degradation,andassemblyofmulti-proteincomplexes.Hdj1,thefirsthumanHsp40/DnaJproteinidentified,playsanimportantroleinproteintranslationandfoldingaswellasinregulationofHsp70function.Hsp40stimulatesATPaseactivityofHsp70whichinturncausesconformationalchangesinunfoldedproteins.
ThisantibodyrecognizesyeastHsp40(Ydj1).Itdoesnotcross-reactwithhuman,mouse,orratHsp40.
Immunoblotting:useat0.5-1ug/ml.Abandof~40kDaisdetected.
Immunoprecipitation
Usershoulddetermineoptimalconcentrationsfortheirapplication.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
QEDBioscience,Inc.10919TechnologyPlace,SuiteC
SanDiego,CA92127
TollFree800.929.2114
Phone858.675.2405
Fax858.592.1509
info@qedbio.com
Visitourwebsiteforadditionalproductinformationandtoorderonline.
qedbio.com