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Hsp60isanabundantproteinsynthesizedconstitutivelyinvariouscelltypesthatisinducedtohigherconcentrationsaftercellshock.Itispresentinmitochondriaofmanymammalianspeciesandhashighlysimilarcounterpartsinbacteriaandplants(whereitislocalizedtochloroplasts).Ingeneral,Hsp60proteinsarepresentinhighconcentrations,areinducedinresponsetoenvironmentalstresses(suchasheatshock)arehomo-oligomericstructuresof7or14subunitsthatdissociatereversIBLyinthepresenceofMg2+andATP,haveATPaseactivity,andplayaroleinfoldingandassemblyofoligomericproteinstructures.Hsp60hasbeenlinkedtoAlzheimer’sdisease,coronaryarterydisease,multiplesclerosis,diabetes,andotherautoimmunediseases.
Antigen:RecombinanthumanHsp60expressedinE.coli.
HostSpecies:MouseAntibodyClass:IgG1Preservatives:0.09%sodiumazideOtheradditives:50%glycerolSPECIFICITYThisantibodyrecognizeshuman,mouse,rat,rabbit,bovine,canine,porcine,ovine,guineapig,hamster,chicken,monkey,Xenopus,andDrosophilaHsp60(60kDa).Itdoesnotcross-reactwithbacterialoryeastHsp60.Theepitoperecognizediswithinaa383-447ofhumanHsp60.
Immunoblotting:useat0.1-1ug/ml.Abandof~60kDaisdetected.
Immunoprecipitation:useat12.5ug/ml
Flowcytometry:useat10ug/ml
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Heat-shockedHeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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