
Anti-Hsp70MonoclonalAntibody
Hsp70genes,membersofamultigenefamily,encodeheat-inducIBLe70kDaheat-shockproteinsthatfunctionasmolecularchaperones.Theyhavebeenidentifiedinmostorganellesofeukaryoticcellsaswellasinbacteria.TheN-terminusofHsp70bindsATPwithhighaffinityandtheC-terminusbindsproteinsandpolypeptides.Hsp70sbindnascentpolypeptidesaswellaspartiallyfoldedintermediatesofproteins,thuspreventingtheiraggregationand/ormisfolding.BindingofATPtriggersaconformationalchangethatresultsinthereleaseofboundprotein.Hsp70sfunctioninproteinsynthesis,proteinfoldingandoligomerization,andproteintransport.
ThisantibodyrecognizesHsp70ofhuman,mouse,rat,rabbit,monkey,guineapig,hamster,chicken,bovine,canine,ovine,porcine,fish,Drosophila,andC.elegansanddoesnotcross-reactwithHsc70.Theepitopeisintheregionofaminoacids436-503ofhumanHsp70.
APPLICATIONSImmunoblotting:1ug/ml.Abandof70kDaisdetected.
ImmunoblottingofHsp70incelllysatesfromhumancancercelllineswith#11096.
Immunohistochemistry:1-10ug/ml
Immunohistochemicalstainingofinflammatorycellsinhumancolontissuewith#11096.
Immunoprecipitation:1-10ug/ml
ELISA:1-10ug/ml
Flowcytometry:10ug/ml
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:HeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.