productdatasheet
Hsp70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides.Hsp70recognizesandbindstonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundprotein.TheABIlityofHsp70toundergocyclesofbindingandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsisthebasisfortheirroleinavarietyofintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.
Thisantibodyrecognizeshuman,mouse,rat,beluga,bovine,canine,fish,guineapig,hamster,monkey,porcine,ovine,andcoralHsp70.
Immunoblotting:useat1:10,000-1:25,000(ECL).Abandof~70kDaisdetected.
Immunoprecipitation:useat1:100dilution
Positivecontrol:HeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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